1 Jan 2020

12th Quarterly Scientific Research article published this week.

Kateb University published it’s yet another scientific journal in the field of Medicine & Health.

Medical science is constantly changing and evolving every-day around the globe and provides new discussions and achievements. That demonstrates the vitality of medical science. In Afghanistan, due to the low level of health and high prevalence of diseases, this creates the need for researchers in various medical fields to conduct applied research on the prevalence of diseases, the use and patterns of medication, useful educational methods for prevention and so on. Therefore, the 12th Quarterly Scientific Research published valuable articles by various researchers in the field of medical sciences. Hopefully medical students could use the result of these studies in their academic progress.

Kateb’s Quarterly Scientific Research Journal welcomes scientific articles by researchers in a variety of fields; particularly in the fields of medical sciences, economics and management, psychology, engineering, computer science and IT, social sciences, law, political sciences and humanities.

Real-time inverse PCR, a substitute and rapid detection method for intron 22 inversion mutation in patients with severe Hemophilia A

Sayed Hamid Mosavi1

 

1- Scientific board member of medicine faculty, Kateb University, Kabul, Afghanistan

Abstract

Hemophilia A (HA) is one of the most deleterious X-linked bleeding disorders in male patients. Early and rapid detection could provide management strategies for this disease. This study aims to improve the classic method for detection of important intron 22 inversion mutations (INV22). Whole blood samples were taken from 21 male children with a history of severe HA, referred to the Iranian comprehensive hemophilia care center. Two groups were involved for detection of INV22 and two methods were examined in a double-blind manner. The first method used a classical method, inverse PCR, and the second method was Real-time inverse PCR. Results showed that both methods could separately detect the INV22 in 11 out of 21 patients with severe HA (52%) in the same accuracy, but with this difference that only one day was needed for detecting of INV22 by Real-time inverse PCR and on other hand 3 days were needed for the classical methods. Because of the medical importance of early detection or prenatal/postnatal diagnosis of this disease, this study recommends the Real-time inverse PCR technique for the rapid detection of this mutation in well-equipped genetic laboratories everywhere.

Keywords: Hemophilia A, Factor VIII, Intron 22 Inversion, Real-time inverse PC

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In vitro spermatogenesis assessment after transplantation of human spermatogonial stem cells

Mahdi Mohaqiq1, Mansoureh Movahedin2, Zohreh Mazaheri3, Naser Amirjannati4

1- Scientific board member of medicine faculty, Kateb University, Kabul, Afghanistan

(Corresponding Author)    

2- Scientific board member of medicine faculty, Tarbiat Modares University, Tehran, Iran.

3- Assistant professor, Basic Medical Science Research Center, Histogenotech Company, Tehran, Iran.

4- Associated professor, Department of Andrology and Embryology, Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran.

Abstract

Background: The introduction of alternative systems in vivo can be very important for cancer patients who are treated with gonadotoxic methods and drugs. In this study, we examine the progression of the spermatogenesis process after transplantation of human spermatogonial stem cells (SSCs) under conditions of testicular organ culture and in vivo condition.

Materials and Methods: Human SSCs were obtained from TESE sample, and the nature of these cells was confirmed by detecting the PLZF protein. These cells, after being labeled with DiI, were transplanted to adult mice testes, treated with Busulfan 40 mg/kg as azoospermia model. Then the culture of host testicular tissue considered as a test group and in vivo transplant as a control group. After 8 weeks, immunohistochemical, morphometric and molecular studies were performed.

Results: The results of morphometric studies indicated that the mean number of spermatogonia, spermatocytes, and spermatids in test groups was significantly lower than the control group (P<0/05) and most of the cells responded positively to DiI tracing. Immunohistochemical in both groups revealed expression of the PLZF, TP1 and Tekt1 proteins in spermatogonial, spermatocyte and spermatozoa, respectively. Also, in human molecular studies PLZF, TP1 and Tekt1, the results showed a significant difference in the expressions of these genes between test groups and control groups (P<0/05).

Conclusion: These results suggest that the conditions of testicular tissue culture after transplantation of SSCs can support the development of spermatogenesis, as well as in vivo.

Key words: stem cells, human, transplantation, culture.

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Assessing health-related quality of life and associated factors on hemophilia patients in Kabul City, Afghanistan

Sayed Mohammad Reza Hosseini1,  Abdul Rahman Hamid2

 

1- Scientific board member of medicine faculty, Ghalib University, Kabul, Afghanistan  (Corresponding Author)

2- Scientific board member of medicine faculty, Ghalib University, Kabul, Afghanistan

 

Abstract

Introduction: Haemophilia is a group of inherited, congenital diseases present all through the life. Those disorders are associated with a chronic burden of morbidity punctuated by episodes of acute deterioration in health-related quality-of-life (HR-QL).  Identifying the factors affecting on quality of life in people with hemophilia in Afghanistan and comparing it with other countries can lead to improved quality of life.

Aid: Assessing health-related quality of life and associated factors on hemophilia patients in Kabul City among people with hemophilia in Afghanistan.

Methods: This is a cross-sectional study conducted among patients with hemophilia in Kabul, Afghanistan. A total of 288 patients with type A and B hemophilia consist of 19 mildly, 83 moderately and 186 severe forms. Patients in the age range of 3 to 42 years and the mean age (13.84 ± 8.63) from 3 centers of hemophilic patients in Kabul include the Esteqlal Hospital, the Children’s Hospital Indra Gandhi and the Afghan Hemophilia Association. HRQoL uses SF-36 through medical records, interviews and self-report collection.

Result: Based on the results Age with all SF-36 subscales except Physical function (P = 0.055) and General health (General health) (P = 0.755), and hemophilia severity with all sub-scales significant relationship was obtained. (p <0.001).

Conclusion: Totally, due to low quality of life in patients with hemophilia, the need to plan for improving quality of life in all aspects patients is necessary.

Keywords: Haemophilia, health-related quality of life, Kabul

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2020-01-29